Investigating the Origins of Fractional ψ-values in Protein Folding Transition States
نویسندگان
چکیده
منابع مشابه
The origins of asymmetry in the folding transition states of protein L and protein G.
Topology has been shown to be an important determinant of many features of protein folding; however, the delineation of sequence effects on folding remains obscure. Furthermore, differentiation between the two influences proves difficult due to their intimate relationship. To investigate the effect of sequence in the absence of significant topological differences, we examined the folding mechan...
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0022-2836/$ see front matter © 2006 E Φ values are experimental measures of the effects of mutations on the folding kinetics of a protein. A central question is what structural information Φ values give about the transition-state of folding. Traditionally, a Φ value is interpreted as representing the ‘nativeness’ of a mutated residue in the transition-state. However, this interpretation is ofte...
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Small single-domain proteins often exhibit only a single free-energy barrier, or transition state, between the denatured and the native state. The folding kinetics of these proteins is usually explored via mutational analysis. A central question is which structural information on the transition state can be derived from the mutational data. In this article, we model and structurally interpret m...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2009
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2008.12.305